At the core of the system is a 5' nuclease assay conducted with the TaqMan¨ PCR Reagent Kit utilizing a fluorogenic probe that has both a reporter and quencher dye attached. When both dyes are ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

Reactions contained 1× Taqman universal master mix (Perkin-Elmer ... s at 95 °C followed by 1 min at 60 °C (1 min 30 s for Alu PCR). Reactions were analyzed using the ABI Prism 7700 sequence ...

This innovative kit allows for the rapid detection of bacterial and fungal contamination within a mere 3 hours, leveraging the accuracy and reliability of real-time PCR technology ... High Specificity ...

Users can search the database by gene, species or type of assay (TaqMan, SYBR Green I or RT-PCR) to find the primer's location, sequence and original reference. Similarly, the RTPrimerDB database ...