Medscape2d
Fresh Embryo Transfer May Be the Best Option for Women With Low IVF Odds
The live birth rate was lower for women with low odds for IVF success when all embryos were frozen and transferred later than ...
eLife12d
Endosomal-lysosomal organellar assembly (ELYSA) structures coordinate lysosomal degradation systems through mammalian oocyte-to-embryo transition
Thereafter, oocytes were incubated with PVA/PBS containing primary antibody overnight at 4 °C. After incubation with a secondary antibody in PVA/PBS for 2 h, oocytes were fixed in 4% PFA and 2.5% GA, ...
jmg.bmj9d
Biallelic variants in α-tubulin isotypes cause female infertility characterised as recurrent preimplantation embryo arrest
Variants of the β-tubulin subunit gene, TUBB8, cause oocyte ... with primary antibodies (HA antibody, Invitrogen, MA-27915 or GAPDH antibody MA5-15738) at 4°C overnight, followed by incubation with ...
BMJ9d
Association of urinary bisphenol A concentrations with in vitro fertilisation outcomes: a systematic review and meta-analysis protocol
The number of oocytes retrieved: the number of oocytes obtained in ... have sufficient studies to pool together to conduct a meta-analysis for some of the primary and secondary outcomes. Although ...
Frontiers9d
Spermatogenic cell-specific SPACA4 is essential for efficient sperm-zona pellucida binding in vitro
Fertilization is a complex and highly regulated process that involves a series of molecular interactions between sperm and oocytes. However ... by incubation with horseradish peroxidase-conjugated ...
jmg.bmj23d
Dosage compensation of the mammalian X chromosome influences the phenotypic variability of X-linked dominant male-lethal disorders
5 To draw these conclusions, the expression profile for the assayable genes on the human X chromosome was determined using a combination of mouse–human somatic cell hybrids and allele specific assay ...
Frontiers9d
The effect of calcitriol on the development and implantation capacity of embryos from hyper-stimulated mice
Therefore, this study was aimed at investigating the effect of vitamin D administered at the time of follicle and oocyte development ... with 1:150 diluted Alexa Fluor 488 goat anti-rabbit IgG ...
BMJ9d
Assessment and support of children and adolescents with gender dysphoria
A marked incongruence between one’s experienced/expressed gender and primary and/or secondary sex characteristics ... but also suspends gonocyte maturation. Oocytes remain dormant and spermatogenesis ...
Science Daily13d
Unveiling the critical role of the exocyst complex in mouse oocyte growth
Communication between oocytes and granulosa cells is essential for oocyte formation, dormancy, reawakening, and maturation. Researchers have demonstrated that a protein complex called the exocyst ...