Reactions were performed in 25 μl aliquots on a 96-well plate (Sigma). Primers for 18S were used as an internal reference. Reactions contained TaqMan PCR master mix (Applied Biosytems), 900 nmol ...

Salmonella, a well-known food-borne zoonotic pathogen, is the causative agent of Salmonellosis affecting public health in both developed and developing countries. Traditional Salmonella detection ...

New techniques in quantitative real-time PCR offer enhanced possibilities for the diagnosis of bacterial diseases, while facilitating rapid assessment of antibiotic resistance in bacterial species.

Introduction This review will focus on recent advances in the development of quantitative real-time PCR (qPCR)-based diagnostic tools allowing detection and optional quantification of bacterial DNA in ...

Real-time PCR as a Diagnostic Tool for Bacterial Diseases Max Maurin Disclosures Expert Rev Mol Diagn. 2012;12 (7):731-754. Five-year View ...

Whether antiretroviral therapy (ART) is always completely suppressive, or HIV might continue to replicate at low levels despite ART in some people with HIV (PWH), is still debated. Here, we ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

Antiretroviral therapy (ART) initiated in the acute phase of HIV infection (AHI) results in a smaller viral reservoir. However, the impact of early HIV-specific T-cell responses on long-term reservoir ...

Multiplex PCR is becoming widely accessible and is being effectively integrated into clinical and laboratory procedures as an automated process. Credit: Seegene “Infectious diseases don’t ...

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The study highlights that a diet rich in fibre, vegetable protein, and fish oil, consumed during gestation and lactation, enhances maternal health by improving intestinal function, mucosal immunity, ...