6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre-amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.