PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre-amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix ...

Two types of COVID-19 tests, the rapid antigen test and the polymerase chain reaction (PCR) test, are available in the United States. The PCR typically relies on lab testing and is still considered ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

View Full Profile. Learn about our Editorial Policies. Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA ...

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...