The quadruplex TaqMan MGB fluorescent quantitative PCR is favored because it can detect multiple target sequences in a single experiment. However, as the number of primers and probes increases, there ...

In the quantitative TaqMan real-time PCR assay, the LOD and the limit of quantification were eight and 80 haploid genome copies, respectively. In addition, three mixed maize samples with known MON863 ...

At the core of the system is a 5' nuclease assay conducted with the TaqMan¨ PCR Reagent Kit utilizing a fluorogenic probe that has both a reporter and quencher dye attached. When both dyes are ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

Detection of meningococcal serogroups A, B, C, W, X and Z was confirmed The detection limit for dry nasopharyngeal swabs was below 2 genomic copies per µl No non-specific amplification was observed in ...

NEW YORK – Roche last week launched a respiratory virus test that detects 12 targets using Temperature-Activated Generation of Signal (TAGS), a new PCR technology developed at the company. The Cobas ...

Methods: A multiplex real-time PCR assay using TaqMan probes was developed to simultaneously detect PEDV, PoRV, and PDCoV. To assess the efficacy of the established assay, 30 clinical samples with ...

Polymerase chain reaction (PCR) is the most commonly used method for nucleic acid amplification due to its ability to detect trace levels of target molecules. However, the development of multiplexed ...

Target-specific end-point fluorescence signals generated by subsequent digital TaqMan PCR can be readily used to identify multiple targets of interest. We demonstrate the dLiNC PCR by detecting 10 ...

For automated workflows, all TaqPath ProAmp master mixes have 72-hour pre-PCR benchtop stability. TaqPath 1-Step RT-qPCR Master Mixes for RNA and virus detection ...