In the quantitative TaqMan real-time PCR assay, the LOD and the limit of quantification were eight and 80 haploid genome copies, respectively. In addition, three mixed maize samples with known MON863 ...

Quantitative PCR with no gel? Impossible! Now with the ABI PRISM 7700 Sequence Detection System, real time PCR has become a reality. Combining a novel chemistry with a fully integrated PCR ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B ...

The Temperature-Activated Generation of Signal (TAGS) technology was developed by Roche for its high-throughput PCR-based instruments.

Herein, we describe a digital Ligation-eNabled fluorescence-Coding PCR (dLiNC PCR) that achieves high-dimensional multiplex nucleic acid detection by employing standard digital TaqMan PCR with a pair ...

Target-specific end-point fluorescence signals generated by subsequent digital TaqMan PCR can be readily used to identify multiple targets of interest. We demonstrate the dLiNC PCR by detecting 10 ...

Sequencing of partial 3D genes from 13 positive samples and the following phylogenetic analysis demonstrated the reliability of the assay. In conclusion, the newly developed TaqMan-based real-time ...

For automated workflows, all TaqPath ProAmp master mixes have 72-hour pre-PCR benchtop stability. TaqPath 1-Step RT-qPCR Master Mixes for RNA and virus detection ...

The QualTrak comprehensive suite of qPCR tools is comprised of a premium selection of trusted Applied Biosystems solutions, including QuantStudio 6 and 7 Pro Real-Time PCR Systems and TaqMan and ...