Real-time TaqMan-PCR was assembled in qPCRBIO Probe Blue Mix (catalog No. 17-514; Genesee Scientific Corporation) or TaqMan Fast Advanced Master Mix (catalog No. 4444554; Thermo Fisher Scientific) ...

The optimization results from the matrix method showed that the optimal reaction system for quadruplex TaqMan MGB fluorescent quantitative PCR was 25 μL: 12.5 μL of 2×Fast One Step Probe RT-qPCR Mix ...

In the quantitative TaqMan real-time PCR assay, the LOD and the limit of quantification were eight and 80 haploid genome copies, respectively. In addition, three mixed maize samples with known MON863 ...

Quantitative PCR with no gel? Impossible! Now with the ABI PRISM 7700 Sequence Detection System, real time PCR has become a reality. Combining a novel chemistry with a fully integrated PCR ...

Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...

The multiplexing capabilities of TaqMan assays are "limited," he said, as standard tests use five fluorescent dyes — one control and four for the target DNAs being measured. The TAGS technology was ...

Methods: A multiplex real-time PCR assay using TaqMan probes was developed to simultaneously detect PEDV, PoRV, and PDCoV. To assess the efficacy of the established assay, 30 clinical samples with ...

Polymerase chain reaction (PCR) is the most commonly used method for nucleic acid amplification due to its ability to detect trace levels of target molecules. However, the development of multiplexed ...

Target-specific end-point fluorescence signals generated by subsequent digital TaqMan PCR can be readily used to identify multiple targets of interest. We demonstrate the dLiNC PCR by detecting 10 ...

For automated workflows, all TaqPath ProAmp master mixes have 72-hour pre-PCR benchtop stability. TaqPath 1-Step RT-qPCR Master Mixes for RNA and virus detection ...